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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been claimed but specific nucleotide sequence info just isn't yet available.
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Blocking peptides are peptides that bind especially to your focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guidebook outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This information outlines additional feasible optimization of mobile and immunoassay parameters to make sure the absolute best final results are...
Antibodies sure to the blocking peptide now not bind towards the epitope about the concentrate on protein. By evaluating the staining from your blocked antibody compared to the antibody alone, one can see which staining is certain.
Concentrate on Details Cyclin E belongs towards the remarkably conserved cyclin family members, whose members are characterised by a remarkable periodicity in protein daftar thr77 abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which add on the temporal coordination of each mitotic party. Cyclin E kinds a complex with and features like a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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